利用反预报方法从截面预言近滴线丰质子核的结合能

在Origin等数据处理软件中,当实验数据较少时,自由参数的不同初始化设置会导致较大的结果差异,这为物理结果的确定带来较大不确定性.通过最小二乘法分析了345MeV/u ~(78) Kr+~9Be反应中产生的丰质子同位素的截面和结合能,并得到线性回归方程.通过回归方程,利用结合能预报部分丰质子核素的截面,以及通过实验截面对近质子滴线核素的结合能进行反预报测量.这对于近质子滴线的丰质子核素实验测量具有较好的借鉴意义.     

四阶段三层次洗毛机理的研究

根据国产原毛表面各种污染物的特点、分布状态及其去除过程，提出了原毛表面污染物去除过程的四个阶段：润湿阶段、溶胀阶段、快速去污阶段和慢速去污阶段；原毛表面污染物可分为附着层、混凝层和结合层。各层污染物在纤维表面附着状态不同、结合形式不同，因而各污染物洗涤难易程度和去除机理各不相同．结合层污染物较难去除，洗涤时针对结合层污染物特点需采用特殊工艺。     

YN相互作用和轻超核∧^5He结合能的计算

在手征SU(3)夸克模型基础上，用共振群方法，给出∧N相互作用的非定域位，并用于轻超核∧^5He的结合能的计算，得到了与实验和其他方法相近的结果．     

Family I.3 lipase: bacterial lipases secreted by the type I secretion system

Based on the classification of bacterial lipolytic enzymes, family I.3 lipase is a member of the large group of Gram-negative bacterial true lipases. This lipase family is distinguished from other families not only by the amino acid sequence, but also by the secretion mechanism. Lipases of family I.3 are secreted via the well-known type I secretion system. Like most of proteins secreted via this system, family I.3 lipases are composed of two domains with distinct yet related functions. Recent years have seen an increasing amount of research on this lipase family, in terms of isolation, secretion mechanism, as well as biochemical and biophysical studies. This review describes our current knowledge on the structure-function relationships of family I.3 lipase, with an emphasis on its secretion mechanism. Received 18 April 2006; received after revision 3 July 2006; accepted 24 August 2006     

Pharmacological versus binding analysis of receptor systems: How do they interplay? Myometrial cell receptors for oxytocin as a paradigm

Summary Binding studies in various biological systems frequently indicate the presence of several binding sites for a biologically active ligand. They differ in their affinity for the ligand in question, binding capacity, and Hill coefficient, which suggests differences in the mechanisms of the binding site-ligand interactions. Identification of the true receptors (sites initiating a cellular response) appears to be difficult. Three clusters of binding sites for oxytocin were found on rat myometrial cells. The oxytocin receptor seems to be linked to the medium-affinity site; the cooperation between the high-and medium-affinity sites in eliciting the uterotonic response seems likely, but lacks experimental proof. Dose-response analysis in partially irreversibly inhibited uterus preparations, the method of equipotent doses (Furchgott-Bursztyn method), and structure-activity analysis of oxytocin-like peptides acting as competitive inhibitors of oxytocin, turned out to be suitable for pharmacological analysis of this receptor system.     

Neuropeptide Y receptors in a protozoaStylonychia mytilus

Radio ligand binding assays(RLBA) were used to study neuropeptide Y (NPY) receptors in a protozoa Stylonychia mytilus. The experimental results showed that 2-3×10+3/mL Stylonychia cells incubated in Pringsheim solution which contained 3H-NPY could specifically bind 3H-NPY and concomitantly present saturable characteristic. This suggested that Stylonychia possessed some specific binding sites for NPY. Scatchard transformations of binding assay for the NPY receptors at 25℃ are compatible with the specific activity of 42.47 fmol/10+3 cells and the binding equilibrium constant of 0.113 nmol/L. The data of 125I-NPY binding assay to the membrane protein extract of Stylonychia indicated that there was a significant difference between the amount of total bound and nonspecific bound of 125I-NPY. This result indicated that NPY receptors were probably localized mainly on the cell membrane.     

强非线性动力系统的两项谐波法

经典摄动法等难以求解强非线性问题,主要局限在于不合理的常频率假设。提出了一类强非线性动力系统的两项谐波法,采用Ritz-Galerkin法,将描述动力系统的二阶常微分方程,化为以频率、振幅为变量的非线性代数方程组,考虑初始条件补充约束方程,构成频率、振幅为变量的封闭非线性代数方程组。利用Maple程序可以方便地求解。分析了一个五次强非线性方程,实例表明,两项谐波法方法简单,具有较高的精度。两项谐波法将谐波平衡法与等效线性化方法相结合克服了二者的缺点吸取了二者的优点,取较少的谐波数目就可以达到比较高的精度。     

DNA与中性红相互作用电化学和光谱研究

利用电化学和光谱方法研究中性红和DNA的相互作用,根据其循环伏安曲线和紫外-可见光谱,在pH=7.05缓冲体系中中性红与DNA发生了嵌插作用.